Molecular Detection of Arcobacter in Human Stool Samples Using Housekeeping Genes
نویسندگان
چکیده
منابع مشابه
Flow cytometric detection of Cryptosporidium oocysts in human stool samples.
Cryptosporidium parvum is an important pathogen that causes diarrhea in virtually all human populations. Improved diagnostic methods are needed to understand the risk factors, modes of transmission, and impact of cryptosporidiosis. In the present study, we fluorescently labeled and counted C. parvum oocysts by flow cytometry (FC) and developed a simple and efficient method of processing human s...
متن کاملMolecular Diagnosis of Strongyloides stercoralis Infection by PCR Detection of Specific DNA in Human Stool Samples
BACKGROUND Strongyloidiasis is mostly an asymptomatic infection and diagnosis of latent infections is difficult due to limitations of current parasitological and serological methods. This study was conducted to set up a PCR-based method for molecular diagnosis of Strongyloides stercoralis infection by detection of copro-DNA in stool samples. METHODS A total of 782 fresh stool samples were col...
متن کاملA PCR-Based Molecular Detection of Strongyloides stercoralis in Human Stool Samples from Tabriz City, Iran
Strongyloides stercoralis is a nematode causing serious infections in immunocompromised patients. In chronically infected patients, the low parasitic content as well as the resemblance of the larvae to several other species make diagnosis basedonmorphology difficult. In the present study, a PCR-based method targeting the internal transcribed sequence 2 (ITS2) of the rDNA region was examined for...
متن کاملHuman housekeeping genes are compact.
Genome Biol. 2, 1018 13 Gray, M.W. et al. (1999) Mitochondrial evolution. Science 283, 1476–1481 14 Lang, B.F. et al. (1999) A comparative genomics approach to the evolution of eukaryotes and their mitochondria. J. Eukaryot. Microbiol. 46, 320–326 15 Henze, K. and Martin, W. (2001) How do mitochondrial genes get into the nucleus? Trends Genet. 17, 383–387 16 Chinnery, P.F. (2003) Searching for ...
متن کاملMolecular Detection of Zonula Occludens Toxin (zot) Genes in Vibrio Cholerae O1 using PCR
Background: Zot (Zonula occludens toxin) is one of the secretion toxins of Vibrio cholerae in small intestine that binds to certain receptors in the epithelial cells and causes a change in the structure of tight junction. The purpose of this research is rapid detection of zot enterotoxin gene using PCR. Materials and Methods: The genomic DNA was extracted by DNA isolation kit and gene amplific...
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ژورنال
عنوان ژورنال: Avicenna Journal of Clinical Microbiology and Infection
سال: 2020
ISSN: 2383-0298,2383-0301
DOI: 10.34172/ajcmi.2020.17